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Of the 39 ingredients that make up a Twinkie, only one of them is strictly a preservative or rather its only purpose in being included is because it’s a preservative.  Some of the other chemicals have preserving side effects, but their use is primarily as substitutes for the dairy ingredients.  The lack of these dairy ingredients and the air tight plastic wrap are the primary reason that the Twinkie can last the 25 days on the shelves and can out last so many of its other baked brethren in that respect.